About: Required experience and knowledge: Experience with nanoflow LC Experience with the orbital trap from Thermo Scientific -Orbitrap Knowledge of LC/MS maintenance Experience with proteomic sample preparation Experience with the following tools for evaluating proteomic MS data - Spectronaut, MaxQuant, DiaNN, Skyline, Perseus Experience with targeted peptide quantification Experience with absolute peptide quantification Experience with phosphoproteome analysis Experience with service measurement of proteomic samples Knowledge of proteomic experiment design Ability to advise users and design user tailored experiment Processing of measured data, statistical evaluation of results, and user reporting       Sponge   Distinct   Permalink

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  • Požadujeme: zkušenosti s nano flow LC, s orbitální pastí od firmy Thermo - Orbitrap, s přípravou a servisním měřením proteomických vzorků zkušenost s následujícími nástroji pro vyhodnocení proteomických MS dat: Spectronaut, MaxQuant, DiaNN, Skyline, Perseus zkušenost s cílenou + absolutní kvantifikací peptidů a analýzou fosfoproteomu znalost údržby a základního servisu LC/MS sestavy; designu proteomického experimentu; výběr způsobu a metody měření uživatelských vzorků a zpracování naměřených dat, statistické vyhodnocení výsledků, reportování uživateli (cs)
  • Required experience and knowledge: Experience with nanoflow LC Experience with the orbital trap from Thermo Scientific -Orbitrap Knowledge of LC/MS maintenance Experience with proteomic sample preparation Experience with the following tools for evaluating proteomic MS data - Spectronaut, MaxQuant, DiaNN, Skyline, Perseus Experience with targeted peptide quantification Experience with absolute peptide quantification Experience with phosphoproteome analysis Experience with service measurement of proteomic samples Knowledge of proteomic experiment design Ability to advise users and design user tailored experiment Processing of measured data, statistical evaluation of results, and user reporting (en)
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